Ko nga peptides kua whakarereketia te methylation, e mohiotia ana ko te methylation-recognized peptides, he pūmua hei whakapaipai i muri i te whakamaoritanga (PTM) me te whai waahi nui ki te whakahaere i nga mahi katoa o te koiora i roto i nga pūtau.Ka whakakorikoria nga pūmua e te methyltransferase ki te whakawhiti i nga roopu hydroxyl ki nga toenga waikawa amino motuhake mo te here kovalent.Ko te Methylation he tukanga whakarerekētanga ka taea e te demethylases.
Kua whakaatuhia e nga rangahau ko nga waikawa amino methylated/demethylated noa ko te lysine (Lys) me te arginine (Arg).Kua kitea e nga rangahau he maha nga mahi koiora o te histone lysine methylation penei i te tiaki me te wehenga o nga pūtau kakau, te korenga o te chromosome X, te whakaritenga tuhinga me te whakautu kino DNA.", i te nuinga o te wa ka pa ki te chromatin condensation me te peehi i te whakaaturanga ira."Ko te histone arginine methylation he mahi nui ki te whakahaere i te whakamaoritanga ira, ka pa ki nga momo tukanga whaiaroaro i roto i nga pūtau, tae atu ki te whakatikatika DNA, te whakawhiti tohu, te whanaketanga pūtau, me te mate pukupuku.Na reira, kua tino whakawhanakehia e Guopeptide Biology te hangarau o te methyl decorative peptides, he mea whakarereke e nga kaiputaiao i muri i te whakamaoritanga pūmua (PTMS) hei awhina i te rangahau.
Whakarereketanga Methylation (Me1, Me2, Me3)
Fmoc-Lys(Me,Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) Ko te 2-OH.HCl(asymmetrical), F i whakamahia moc-Arg(me)2-OH.HCl(hangarite) me etahi atu rauemi mata i hangaia e te tukanga whakahiato-waahanga FMOC kia whiwhi Lys me Arg methylated peptides, me nga hua. i purea e HPLC.Ko te hiranga papatipu e tika ana, ko nga chromatograms HPLC me te COA kua whakaratohia mo te hua kua oti.
Te wa tuku: Noema-24-2023